Coco Fusco Cultural Commentary Essay Example for Free

Coco Fusco Cultural Commentary Essay This performance was intended to mock Western concepts of the exotic but instead took on a different facade when most audiences did not realize it was a performance piece. Their cage became â€Å"a metaphor for [their] condition, linking the racism implicit in the ethnographic paradigms of discovery†[1]. Reactions and commentary received throughout a span of two years allowed Coco Fusco to gage an even stronger sense of â€Å"otherness† where she was looked upon as a specimen instead of a human being. Being dehumanized in such a form cannot be easy to handle even when taking into account the fictional situation she and Gomez-Pena were in. However, the prevalent â€Å"otherness† for Coco Fusco wasn’t exclusive to the performance piece; as a Cuban-American she had already encountered that denial of one’s actual presence within society. As a young child her family hid the reasons for and meaning of comments/looks made as a way of protecting her from the harsh realities. Even in later years when Coco Fusco returned from her study abroad trip to Paris, her family was exuberant by the thought of her speaking French. She anecdotes thinking that her â€Å"newly acquired French impressed everyone much more than [her] English ever had†[2]. This inadvertently established that languages of the Western world were superior to her vernacular Spanish. The implication was that if she relinquished the use of Spanish or even the hybrid Spanglish she would be more successful in life. In a reading covered by Professor Alba-Salas the author reaffirms this notion by saying that â€Å"those who achieve success have done so within social and educational systems that favor the use of English over Spanish†[3]. Coco Fusco’s family was consumed by the same fear most immigrant families have; the fear of being marginalized as the â€Å"other† and never really reaching the viable potential one can have. Soon after Coco Fusco comes to realize the immense â€Å"culture clash† she had been living. She realizes the implications of those stares and the thoughts brought upon by her speech. It is then when she takes in upon herself to at least attempt to dispel or even clarify the misconceptions of the population through her performances. In the Two Undiscovered Amerindians performance it is clearly seen how two conflicting cultures create animosity where the privileged, in this case the audience, attempt set the standard for the normative. Coco Fusco notes that as she and Gomez-Pena assume their â€Å"stereotypical role of domesticated savage[s] and many audience members [feel] entitled to assume the role of the colonizer†[4] where as to continue the already rampant cycle of discrimination. After a particular performance one young woman came back to share her discontent, claiming that Gomez-Pena was â€Å"ungrateful for all the benefits he had received thanks to multiculturalism†¦ Gomez-Pena responded that multiculturalism was not a gift from the white but a result of decades of struggle by people of color†[5]. In this discourse Gomez-Pena reinforces the idea of this â€Å"culture clash† in America and how the privileged still see themselves as that standard that enables them to pass judgment. Nevertheless both â€Å"culture clash† and this sense of â€Å"otherness† within communities creates a harmful effect in society but equally harmful is the alienation that appears as an outcome of their presence. The other tends to feel this alienation most when realizing there is disengagement between them and their society, work, and even at times themselves.

Tumour Supressor Genes and Retinoblastoma

Tumour Supressor Genes and Retinoblastoma Throughout our life time growth is constant. Barring a few cells, most cells continue to grow, divide and replace themselves. Such processes require a strict regulation and this is brought about by cell cycle processes. These processes are carried out by proteins which control the growth and development. Proteins are coded by the genes and when the genetic information is altered or is mutated, it leads to a protein which is dysfunctional and the cell might lose its restraint on growth processes. This in turn leads to an uncontrolled growth of the cells which then causes cancer. Cancer is a disease of the genes. At a molecular level, there are two major reasons which have been attributed for cancer induction. The first one is the gain of function in genes and the other one is loss of function of genes. The genes which gain function, that is, get activated after certain specific events are known as proto-oncogenes. They are typically activated in cancer cells conferring new properties, such as hyperactive growth and division, protection against programmed cell death, loss of contact inhibition between cells, and the ability to become established in diverse and adverse environments. Those genes which lose their functions due to specific events are termed tumor suppressor genes (Eeles et al 2004). Tumor suppressor genes are inactivated in cancer cells, resulting in loss of normal functions, such as accurate DNA replication, cell cycle control, adhesion within tissues. Tumor suppressor genes are named such because when they are expressed in a normal cell they maintain the cell in a differentiated state and do not allow unchecked proliferation of cells. Therefore, at least one functional copy is required to prevent tumor initiation. That there are tumor suppressor genes was proposed when it was experimentally found by Harris et al. (1969) in cell fusion experiments wherein a fusion between a normal and a tumor cell rendered the cell non-cancerous and therefore, it was proposed there must be tumor suppressor genes which suppress cell growth in a dominant fashion (Skapek et al, 1997). As long as the normal copy is functional, the protein expressed maintains normal functioning of the cells. Many tumor suppressors have been identified and extensively characterized. TP53, WT1, NF1, BRCA, VHL, APC, MEN1 are a few examples of tumor suppressor genes. Tumor suppressor genes are divided into three categories- the gatekeepers, the caretakers and the landscapers. Gatekeepers are genes that directly regulate the growth of tumors by inhibiting their growth or by promoting apoptosis. In contrast, inactivation of caretakers does not directly promote growth of tumors. Rather, inactivation of caretakers leads to genetic instability that only indirectly promotes growth by causing an increased mutation rate. Landscaper genes do not directly affect cancer cell growth but contribute to an abnormal stromal environment that contributes to neoplastic transformation of the overlying epithelium. This project deals with one gatekeeper gene, known as retinoblastoma susceptibility gene (RB1). RB1 is the first tumor suppressor gene to be identified and cloned (Lee at al., Eeles et al, 2004; Andrade et al, 2006). It is the gene which when mutated predisposes a person to a common malignancy of the eye, retinoblastoma. Retinoblastoma is the most common intraocular cancer in children. It generally manifests in children before the age of five years with a majority of the tumors occurring by the age of 2 years (Lee et al., Eeles et al, 2004). Clinical features of retinoblastoma: As the name implies, retinoblastoma is one of the rare embryonic neoplasms originating in the retina. It is the most common intraocular tumor in children (Valverde et al, 2005). It was first described as a specific entity by James Wardrop. In majority of cases, the first sign at presentation is the characteristic cats eye reflex, which is usually noted by direct visualization. This white, pink-white, or yellow-white pupillary reflex, termed leukocoria, results from replacement of vitreous by the tumor or by a tumor growing in the macula. Another common symptom, strabismus or squint (exotropia or esotropia) can occur alone when small macular tumors interfere with the vision, or can be associated with leukocoria. Uncommon presenting signs for retinoblastoma are red, painful eye with secondary glaucoma, orbital cellulitis (infection of the soft tissues of the eyelids), unilateral mydriasis (excessive dilation of the pupil due to disease or due to trauma), and heterochromia (the iris of the two eyes show different color). In rare cases, presenting symptoms include hypopyon (pus in the anterior chamber of eye), hyphema (blood in the anterior eye chamber), keratitis, and vitreous hemorrhage (Vogelstein and Kinzler, 2002). A complete evaluation for retinoblastoma includes ophthalmologic examination, radiographic evaluation (skull X-ray, CT scan, MRI), and more recently, genetic testing. Fundus examination of first degree relatives is also done to look for the presence of retinoma or a regressed tumor which may indicate a hereditary component of the disease. Retinoblastoma can be exophytic or endophytic. It is exophytic when tumor occurs between the choroid and the retina and it is endophytic when it extends from retina towards the vitreous chamber. Therapy depends on the stage at which it is discovered. Based on that, treatment is given which includes enucleation, external beam radiotherapy, cryotherapy, episcleral plaques, xenon and argon laser photocoagulation, and chemotherapy. The choice of treatment depends on the factors such as: Multifocal or unifocal disease, Site and dimensions of the tumor, Diffused or focal vitreous seeding, Age of the individual, Histopathological finding. Therefore, staging and grouping of the disease is very important. Retinoblastoma can manifest in one of the eyes, both eyes and / or pineal body of the brain. When it is observed in one eye, it is referred to as unilateral retinoblastoma. When it is present in both the eyes, it is referred to as bilateral retinoblastoma. It is called trilateral retinoblastoma when the pineal gland is also involved. Why should retinoblastoma occur in one eye in some individuals and both the eyes in others? One more observation was that most of the individuals who came with retinoblastoma in both the eyes were diagnosed at an age earlier than those who presented with unilateral retinoblastoma. What is different in the genotypes of these two types of individuals that warrant a delayed onset of disease in the unilateral retinoblastoma cases? This was explained by Knudson in his study. Genetics in retinoblastoma: Retinoblastoma occurs with a frequency of 1 in 13,500 (Mateu et al., 1997) to1 in 20,000 (Di Commo et al., 2000). It is seen in both hereditary and nonhereditary forms (Knudson, Lee et al.) and shows no significant variation between races, countries, or level of industrialization (Mateu et al., 1997). However, contradictory views have been expressed by Mastrangelo as he questions the credibility of the data. Previous studies indicated that a gene predisposing to retinoblastoma manifests in young children was localized to chromosome 13. The gene was probably at band q14, since several retinoblastoma patients had shown to carry constitutional deletions of this region of chromosome 13. Retinoblastoma can be hereditary as well as sporadic. Those who have a mutation at one of the alleles of RB1 in germline cells are said to be predisposed to the disease. Such cases are categorized under hereditary retinoblastoma. Both hereditary as well as non-hereditary forms of retinoblastoma may show tumors in one or both the eyes. In the hereditary form of retinoblastoma, a germline mutation is transmitted as high penetrance (90%) autosomal dominant trait (Martinez et al). Most reported cases are sporadic (Knudson, 1971; Mateu et al, 1997). Retinoblastoma is caused by two mutational events at the retinoblastoma (RB1) locus (Knudson, 1971). That is, biallelic inactivation of RB1 due to mutations is a crucial event in the development of retinoblastoma (Andrade et al, 2006). Knudsons hypothesis: Alfred Knudson realized the implications of the fact that individuals with hereditary bilateral retinoblastoma were diagnosed at a younger age than those children with non-heritable disease, mostly unilateral retinoblastoma. The mean age of individuals on diagnosis was found to be 19 months in unilateral retinoblastoma cases whereas it was found to be 5 months in bilateral retinoblastoma cases. Also, in most cases of familial retinoblastoma, tumor develops in both the eyes. To explain these two important phenomena, age of onset and tumor development (unifocal or multifocal) in one or both eyes, Knudson proposed the two hit hypothesis. The hypothesis seeks to explain the occurrence of hereditary and nonhereditary retinoblastoma and its correlation with the age of onset of the disease. According to Knudson, the first mutational hit can be inherited through the germline or can be somatically acquired, whereas the second occurs somatically in both cases and leads to tumor in cells that is double defective at the RB1 locus (Knudson, 1971; Mateu et al., 1997). Thus in case of hereditary retinoblastoma, the individual must have inherited the mutation through the germline and hence, during the course of development may show the second mutational hit leading to tumorigenesis. As this individual already has a mutation in germline, it shows the second hit early in the life time. Statistical analyses indicated that as few as two mutational hits were rate limiting for the development of retinoblastoma tumors. The occurrence of the first mutation (M1) in the germline and all developing retinal cells gives retinoblastoma tumor a head start in hereditary cases (only M2 must arise in a retinal cell), compared to non-hereditary tumors where both M1 and M2 must arise in a single retinal cell. On the other hand, in unilateral retinoblastoma cases, the individual has not inherited a germline mutation at the RB1 locus. During the course of development, the cells of such patients gather both the mutations in somatic cells with some exceptions. Thus these tumors arise later than the hereditary forms of retinoblastoma. Knudson performed a statistical analysis of some patients presenting with retinoblastoma. The number of tumors in each eye was calculated and he devised a distribution keeping number of tumors in one eye, m= 3. Each tumor which is seen originates from a single cell. Thus, a mutation rate can be roughly calculated. If the total number retinal cells are n, m/n is the probability of a cell undergoing mutation at one of the alleles. Now, retinoblastoma is derived from the inner and outer neuroblastic layer. The order of magnitude of retinoblasts is reflected by the magnitude of the number of ganglion cells which are derived from the early differentiated inner nuclear layer of the retina. The estimated number of ganglion cells has been put at 2 x 106 per retina. Thus, using this as an approximation for the total number of cells, the probability that a cell will inherit one mutation is 0.75 x 10-6. Since a majority of hereditary cases occur in the first two years of life, the probability expressed per year at either member of the autosomal gene pair would be one fourth of this value, or approximately 2x 10-7 per year. This estimates the rate of second mutation in mutated cells (Knudson, 1971). It is seen that the rate at which the second mutation occurs is relatively lower than the first mutational hit. Retinoblastoma in hereditary cases which present themselves as bilateral retinoblastoma show high penetrance. In such cases, the vast majority of high penetrance mutations are null alleles where the mutations abrogates which destabilizes RB mRNA, presumably due to pre mature truncation of translation, so that no pRb is detected. However, some mutations show low penetrance. The low penetrance phenotype can result from several different types of RB alleles. Germline deletion of the whole RB gene often results in unilateral retinoblastoma, presumably because an unknown adjacent critical gene is also deleted, without which the RB-/- cell cannot survive. Only cells in which M2 is a different intragenic RB mutation on an allele with the adjacent critical gene still intact can survive to form retinoblastoma. Some mutations reduce expression of wild type pRB by targeting the promoter or splice sites. In-frame mutations result in a stable pRB with some aberrant functions. THE RB1 GENE: The RB gene family includes at least three members- RB1, RBL1, and p130. The RBL1 and p130 also show similar protein binding characteristics as RB1 and therefore they come under one gene family (Mulligan and Jacks, 1998). All three genes code for pocket proteins because their main sequence similarity resides in the pocket domain, which mediates interactions with the cellular and viral proteins to exert biological functions of this family (Pogoriler et al., 2006). The RB1 gene has been localized on chromosome 13 of humans on the long arm with locus 14.2 (Cavenee et al, 1971). The RB1 gene codes for a protein which is 928 amino acids long. There are 27 exons in RB1 and occupies nearly 200 kb of the genome. The gene transcribes a 4.7 kb mRNA. The exon size varies across the gene. The largest is exon 27 which is 1892bp long while exon 24 has only 30bp in its exonic region (NCBI database: www.ncbi.nlm.nih.gov/). Comings et al. suggested that RB1 is a tumor suppressor gene which is recessive at cellular level (Comings, 1971; Di Commo et al., 2000). The RB1 promoter present upstream of the exons does not show the typical TATA box binding domain. The promoter sequence of RB1 reveals that the sequence between -300 and +400 is GC rich (Hong et al, 1989). As the promoter lacks a TATA element, it might explain the presence of three transcriptional initiation sites. Deletion analysis of the promoter by Hong et al demonstrated that the sequence stretching from +13 to +83 suffices the promoter activity. Another characteristic of the promoter region is that the G+C rich region shows similarity with many housekeeping genes (Hong et al, 1989). This outlines the fact that the RB1 gene is expressed constitutively in almost all tissues of our body. The Leiden Open source Variation Database (LOVD) maintains the reported mutations in the RB1 gene. Almost 940 mutations and polymorphisms have been reported in RB1 by many scientists (www.rb1-lsdb.d-lohmann.de/). Single base pair mutations are the most frequent M1 mutations and account for nearly 40% of the confirmed mutations, followed by short and large mutations. The second hit might be associated with loss of heterozygosity (LOH) mutations, promoter hypermethylation, or even a second independent base substitution (Andrade et al, 2006; Lohmann et al., 1996). The most common point mutation found in the studies is the change from C>T at the CGA sites which codes for amino acid arginine. This change leads to premature termination of the protein (Lohmann et al., 1996). Expression of Rb protein: Initially, RB gene was considered to be expressed ubiquitously in all tissues of the body considering its role in maintaining cell differentiation (Karantza et al, 1993). However, later studies revealed differential expression of Rb protein. In situ Hybridization studies done with Rb expression during embryogenesis show, that the Rb family of proteins is differentially expressed in only certain specific cell lineages. According to studies, Rb1 mRNA transcripts were detected not only during in the ganglionic cell layer of retina but also during neurogenesis, hematopoiesis, myogenesis, lens development prior to and during differentiation. In the liver and the CNS, RB1 is co-expressed along with p107 protein. Consequently, RB-/-, p107-/- cells undergo cell apoptosis. RB1 transcripts were also detected throughout myogenesis. pRB has also been found to be expressed during spermatogenesis (Yan et al, 2001). Since RB1 mutations specifically arise in the human eye, analysis has been performed for the developing eye. The results suggested that RB1 transcripts were detected in the ganglion cell layer of the developing retina from embryonic day 14 through 18 (Jiang et al, 1997). Preliminary studies had indicated that Rb expression in developing retina initiates as the cells commit to differentiation, but pRb has been detected only in certain subsets of retinal cells (Di Commo et al, 2000). In conclusion, pRb expression is important for terminal mitosis in peripheral nervous system, keratinocytes, and skeletal muscles (Di Commo et al, 2000). THE RETINOBLASTOMA SUSCEPTIBILITY PROTEIN: The RB1 gene transcribes a 4.7 kb mRNA which encodes a phosphoprotein which is 928 amino acids long. It is an example of a pocket protein as there are sites or pockets which interact with other proteins. The protein is a negative regulator of the cell cycle. The pRb migrates in SDS PAGE as a multiple, closely spaced bands with molecular weights between 110 and 114 kDa (Skapek et al, 1997). The pRb can be divided into three domains protease resistant, protease soluble and structural domains comprising of the N terminal, R motif, and A/B pocket (Di Commo et al, 2000). The N Terminal: The N terminal region extends from amino acid 1-379. Although the N terminal is well conserved among Rb orthologs and paralogs, it has been studied far less. However, a significant number of mutations in this region occur in retinoblastomas, strongly implicating it in tumor suppression. Another study by Goodrich et al, 2003, suggested that integrity of the domain is necessary for rescue from both developmental defects as well as tumor susceptibility. The crystal structure of the domain reveals a globular entity formed by two rigidly connected cyclin folds similar to the pocket domains which suggests that Rb evolved through domain duplication. A coherent conformation of the Rb holoprotein has been suggested in which the N terminal domain and the pocket domains interact directly (Hassler et al). The A/B domain: The pocket domain consists of two non consecutive stretches of amino acids, A (amino acids 380-577) and B (amino acids 645-785) (Xiao et al, 2003). It is coded by the exons 12 through 22 of the RB1 gene (Brichard et al, 2006). This region has been shown to interact with many proteins. This domain has been shown to be critical for many interactions of pRb including interaction with a variety of cellular proteins like E2F transcription factor (extensively characterized), tethering of pRb to nuclear structures (Skapek et al., 1997), phosphorylation during the G1 phase of cell cycle. Between the A and B domain is a small stretch of 75 amino acids, the spacer region. A small deletion within the spacer region or replacement of the spacer region with a random sequence has no effect on the function of pRb. However, deletion of entire spacer affects the physical interaction between the two domains. There is another pocket referred to as the C pocket domain in the large A/B domain of the pRb protein. This C pocket lies within the minimal functional domain of RB (Rb amino acids 395 to 876) (Whitaker et al, 1998). The C terminal: The C terminal stretches from amino acid 786 to 928. Of the sixteen sites for phosphorylation by cdks identified, six lie in the C terminal. It has been demonstrated that phosphorylation of S788 and S795 destabilizes the E2F complex interaction directly while phosphorylation of T821 and T826 induces an intramolecular interaction with Rb pocket that destabilizes the remaining interactions indirectly (Rubin et al.). The C terminal also contains a Nuclear Localization Signal (NLS) as well as cyclin binding motif [R/K]XL that are important for Rb phosphorylation (Di Commo et al, 2000). RB AND CELL CYCLE: pRB is a negative regulator of the cell cycle. The cell cycle consists of DNA synthesis (S phase) and mitosis (M phase) separated by two gap intervals, G1 and G2. When they are not cycling, cells are in a quiescent phase, G0, and extra- and intracellular signals are required to re-enter the cell cycle. Serum-starved cells in G0 will enter G1upon growth factor stimulation, and will return to G0 if mitogens are removed prior to a point in late G1. Beyond this restriction point (R) cells traverse through S, G2 and M, and will not stop even if serum is removed. There are two checkpoint controls which ensure proper cell cycle progression. The first one is referred to as the G1S checkpoint that ensures that there are proper environmental conditions for cell division. Importantly, many of the extracellular and intracellular signals that regulate passage through R point converge on the pRB pathway (Di Commo et al.) The second one is called as the G2M checkpoint which ensures that all the req uirements for cell division are fulfilled. Fig: Schematic diagram showing RB phosphorylation pathway. (1) E2F activity repressed in G0 phase imparting cell cycle inhibition. (2) Mitogens trigger a signaling pathway leading to activation of cdk 4/6- cyclin D, cdk 2-cyclin E complexes which in turn phosphorylate RB sequentially rendering it inactive. (3) E2F mediated gene expression allows progression through S phase. (4) During transition to mitosis, the action of mitosis reactivates RB to hypophosphorylated form. The regulation of pRB is by phosphorylation. Hypophosphorylated pRb is in its active form whereas on phosphorylation it loses its activity. The protein contains 16 CDK recognition motifs (S/TP) for phosphorylation, six of which are located in the C-terminus (Rubin et al, 2005). Hypophosphorylated pRB binds target proteins and arrests cells in G1. This block is relieved by a crescendo of CDK-mediated phosphorylation that begins as cells in G1 approach R, and is abruptly reversed at the end of M phase. The major targets for hypophosphorylated pRB are E2F and co repressors such as Histone Deacetylases (HDACs). E2F belongs to a family of transcription factors who have evolutionarily conserved domains including the one for DNA binding and a dimerization domain. E2F is found as a heterodimer with another class of proteins- Differentiation Regulated Transcription Factors (DRTF-b). E2F-pRB co repressor complexes maintain the gene repression (Lees et al, 1993) that is required for progression through R, such as cyclin E. To pass this checkpoint, these repressor complexes are disrupted in two stages by the sequential action of cyclin D and cyclin E activated CDKs. As cells exit G0, cyclin D levels rise, causing activation of CDK4/6 and phosphorylation of multiple C-terminal sites on pRB (Gorges et al, 2008). The C-terminal region of pRB contains a series of (R/K)XL cyclin docking motifs. Ser 795 is the first site to be phosphorylated, and is critical for inactivating growth suppression by pRB. Following D-CDK4/6 phosphorylation, the increase in negative charge promotes an intramolecular interaction between the C-terminus and a series of positively charged lysine residues (the lysine patch) that surround the LXCXE binding groove in the B domain of the pocket. Associated LXCXE proteins, such as HDACs, are also dislodged. However, E2F which does not use LXCXE motif to interact with the pRB does not dislodge. Removing HDACs is thought to relieve active repression of certain target genes such as cyclin E. The genes which are required for DNA replication like gene for thymidine kinase, DNA polymerase and dihydrofolate synthase are also activated as a result of phosphorylation of RB1 and subsequent loss of interaction with E2F protein which now recruits other transcription factors. The cell thus enters the S phase. In the S phase of cell cycle, DNA gets replicated and synthesized. Histone synthesis also occurs during the S phase. Once DNA replication is complete, the cell enters another gap, G2 ÂÂ ­where the cell continues to grow till the cell enters the M phase or mitosis phase. Significant protein synthesis takes place during the G2 phase. Proteins like microtubules involved in mitosis stage are synthesized. At the end of the G2 is the G2/M checkpoint. Once it overcomes the second checkpoint, the cell enters actual cell division- mitosis. RB1 PROTEIN INTERACTIONS IN CELL: Cell cycle control at the G1 S checkpoint and growth suppression as well as differentiation into a particular subset of cell is considered the major function of RB1 protein. Transcription regulation is a control mechanism that is critical for fundamental processes such as cell growth and differentiation. Proteins involved in transcription regulation either bind to DNA sequences or act as co-activators or co-repressors. pRb is one such transcription regulator. In accordance with its role as a tumor suppressor, introduction of Rb into Rb-/- cells diminishes their capacity for malignant transformation which underlines the fact that pRb plays a critical role in DNA replication, cellular senescence, differentiation, and apoptosis, placing pRb at the center of cellular proliferation and tumorigenesis through its interaction with various proteins. At least three distinct protein binding activities have been identified and extensively studied: the large A/B pocket binds E2F (Benevolenskaya et al, 2005), the A/B pocket binds the LXCXE peptide motif, and the C pocket binds the nuclear c-Abl tyrosine kinase (Whitaker et al, 1998). Also, the protein also stabilizes the heterochromatin to maintain the overall chromatin structure. The C pocket is distinct from the A/B pocket domain as pRb can simultaneously bind to c-Abl as well as E2F (Whitaker et al, 1998). The A/B domains are sufficient for E2F subunit binding to Rb. However, the E2F-DP1 heterodimer requires the presence of C terminal. The retinoblastoma protein inhibits E2F mediated transcription via two distinct mechanisms- 1.) pRb binds to E2F transactivation membrane and inhibits E2Fs ability to promote transcriptional activation of E2F dependent genes. 2.) pRb actively represses expression of certain genes by recruiting HDACs and other chromatin remodeling factors (Gorges et al, 2008). The C terminal has also been shown to bind to MDM2. MDM2 interaction with the extreme C terminal region has therefore been shown to contribute towards regulation of apoptosis (Janicke et al., 1996; Sdek et al, 2004). MDM 2 has been known to stimulate E2F transactivation activity and promote S phase entry of cells, independent of p53 (Sdek et al., 2004). The retinoblastoma tumor suppressor protein has been known to bind directly and inhibit a transcriptionally important amino terminal kinase domain of TATA- binding protein associated factor TAFII250 (Siegert et al., 2000). TAF II250 is the largest of approximately 10 TAF subunits of Pol II specific human TFIID. It directly binds to TBP and is believed to be a central scaffold for assembly of TAFs and TBP into a stable TFIID complex (Siegert et al., 2000). The retinoblastoma protein interacts even with transcription factors ATF2. ATF2 enhances the activity of TGF ÃŽÂ ² promoter. The retinoblastoma protein also enhances the stimulatory effects of ATF 2. The site on Rb required for its effect alone and in combination with ATFs has been mapped mainly to A/B products and the C pocket (Li and Wicks, 2001). It has been also reported that the amino terminal of BRCA 1 can efficiently bind to the ABC region (from amino acids 379-928) of the Rb protein. It has been shown that growth suppressor activity of BRCA1 takes place only in presence of a functional Rb protein (Aprelikova et al., 1999). pRb has been known to interact with proto-oncogene c-jun. One of the transcriptional modulator target sites of pRB is the AP-1 binding site within the c-jun and collagenase promoters. c-Jun also physical interacts with pRb where the C terminal site of leucine zipper interacts with pRb (Nishitani et al., 1998). pRb also represses the expression of c-Fos gene the gene product of which , Fos, is one component of the heterodimeric transcription factor, AP-1. Thus, pRb can be functionally linked to c-jun for transcriptional regulation. pRB when recruited to DNA via E2F is a potent transcriptional repressor due to its ability to recruit HDACs (Kennedy et al., 2001) and histone methylases. Again, one pRB binding protein, EID1 is a potent inhibitor of histone acetylases, p300 and CBP and blocks differentiation (Benevolenskaya et al.). HDACs interact directly with pRB by means of a LXCXE motif (Kennedy et al., 2001; Gorges et al., 2008). p53 controls phosphorylation of pRB in a cell indirectly. On DNA damage induces the transcription of its target gene p21WAF1/CIP1. The p21 protein thus formed binds to two different proteins. First, it inhibits the activity of CDKs in cell which in turn keeps pRB in active state. Thus, p53 helps in negative regulationof cell cycle. Second, the p21 protein interacts with the PCNA which then leads to inhibition of cell cycle (Hsieh et al, 1999). Androgen receptors show a unique interaction with pRb at the N terminal. Over expression of pRb leads to increased transcription activity of androgen receptor, AR. pRb also potentiates the activity of glucocorticoid recptors, GR. However, loss of pRb activity inhibits AR but not GR activity (Lu and Danielsen, 1998). MUTATIONS IN RB GENE: After RB1 gene was first cloned by Friend et al in 1986, mutations have been identified in the gene ranging from single base pair deletions, to small length insertions and deletions (Lohmann et al. 1996, Andrade et al. 2006). The mutations do not show any hotspots and they are widely distributed over the 27 exons of RB1 as well as the promoter region (Dalamon et al. 2003). The most common mutation found to result in predisposition to retinoblastoma is the point mutation from C>T. At times the mutation at this point changes the codon from CGA to TGA, from Arginine to stop codon. This premature truncation of protein leads to abrogated expression of pRb in cells. Hypermethylation of RB1 promoter has been also observed as a common epigenetic event in certain tumors (Gonzalez- Gomez et al., 2003). Retinoblastoma Gene Mutation database (RBGMdb) maintains a comprehensive list of all mutations in the RB1 gene. Till now, 932 mutations have been reported in the RBGMdb. Most of the mutations are nonsense mutations (42%). However, if recurrent nonsense mutations are not considered, the percentage comes down to 18%. RB1 mutations are scattered all along the genomic sequence. However, there are a few hotspots which show high recurrence. Most of the recurrences correspond to C>T changes in the sequence. Out of 351 nonsense mutations 271 (79%) are C>T transitions in eleven CGA (arginine) codons, in exon 8, 10, 11, 14, 17, 18, and 23. No mutations have been found in the three other CGA codons in exon 1 and 27. In four of the mutated CGA codons, R251and R255 in exon 8, R451 and R455 of exon 14, a high frequency of constitutive hypermethylation has been demonstrated. In addition to hot spots, frameshift and point mutations leading to translational changes or splice site mutations are scattered along the retinoblastoma coding region and non-coding adjacent splicing sites. With the exception of exons 5, 14, 15, 24, 25 and the non-mutated exons 26 and 27, frameshift mutations are randomly distributed through the RB1 coding sequence. Splicing mutations are also evenly distributed, but show preference for intronic sequences adjacent to exons 6, 12, 16, 17, 19 and 24. It is worth to mention that most missense substitutions (60 %) are located in cyclin box B, underlined by exons 19 to 21.

ICT for Special Educational Needs Support

ICT for Special Educational Needs Support The Potential of ICT Supporting Pupils with Special Educational Needs Chapter 1: Introduction 1.1 Background Information and Communications Technology (ICT) is the use of computers in education and offers enormous potential to teachers and pupils.  There is a growing number of consistent evidence which shows that ICT can and does improve learning outcomes, particularly in the core subjects of English and Mathematics (Cox et al, 2003).  Providing high quality software is matched to the specific needs of the individual, it can act as an effective and powerful tool in learning. While it cannot replace high quality teaching, it can enhance the learning process. The application of ICT to teaching and learning can provide many benefits such as, facilitating communication, increase access to information, improve motivation, increase problem solving capabilities and enable deeper understanding of complex ideas. ICT can provide pupils with special educational needs improved access to learning and areas of the curriculum which may have been previously inaccessible. According to Westwood (2003), â€Å"The largest single group of students with special needs comprises those with general and specific learning difficulties that are not related to any disability or impairment. Estimates suggest that this may be close to 20 per cent of the school population.  These learning difficulties most frequently manifest themselves as problems in acquiring basic literacy and numeracy skills’ which impact adversely on a child’s ability to learn in most subjects across the curriculum.† (Westwood, 2003, P5) The Audit Commission reports that one in five children in England and Wales has Special Educational Needs (SEN). This includes students with serious physical or learning difficulties but also many students whose reading, writing and numeracy skills develop slowly. Special needs include conditions such as dyslexia, physical disabilities, speech and language disorders, visual impairment, hearing loss, difficulties in communication, and emotional and behavioural difficulties.   In recent years, there has been an increase in evidence that technology can help these children overcome their communication and physical difficulties, so that they can be included in lesson activities and access a wider curriculum, as suggested by the Irish body, the Education of Science Department (ESD) in The Learning-Support Guidelines (2000), â€Å"‘Interactive computer-based systems allow the possibility of individualising the educational process to accommodate the needs, interests and learning styles of individual pupils. Individualised planning is fundamental to the successful use of ICT in supplementary teaching as it is to other forms of Learning Support. The planning process would include identifying a pupil’s individual learning needs and considering how ICT might be used to meet those needs.† (ESD, 2000, P86-87) Every learner has an entitlement to all the elements of cognitive, literacy and cultural learning. This belief is generally shared by all working with learners who experience any kind of difficulty, for whatever reason.  The introduction of the national Curriculum and the Code of Practice on the Identification and Assessment of Special Educational Needs (DfE, 1994), superseded by the new Code of Practice (2002), have given teachers the opportunity to put this clearly into practice because they provide and support a curriculum for all.  It is explicit in the National Curriculum that all learners have a right to a broad, balanced and relevant curriculum, which makes it difficult to exclude any learners from this entitlement.  Stansfield (2001) believes that incorporating ICT support strategies can be advantageous in making this occur. â€Å"For learners with Special Educational Needs (SEN), the use of ICT can convert this entitlement to reality.  The National Curriculum makes clear in each subject document that ICT should be used where appropriate, to support this process.† (Stansfield, 2001, P5) The National Curriculum (1999) identifies with this and makes clear in each subject document that ICT should be used where appropriate, to support this process. Appropriate provision should be made for pupils who need to use: Means of communication other than speech, including computers, technological aids, signing, symbols or lip-reading; Technological aids in practical and written work; Aids or adapted equipment to allow access to practical activities with and beyond school (National Curriculum, 1999) In Wales, the government have recently put forward their vision for education for Wales in the 21st Century, with a far stronger emphasis on including all learners and the use of ICT to support this.  The Learning Country: Vision into Action, (DELLS, 2006) highlights the need for a learner-centred curriculum if standards are to be raised and all learners’ experiences of education improved.  The document makes clear that all learners means just that –  including pupils with learning difficulties, specific disabilities and motivation problems; those who are gifted and talented, from different ethnic/cultural groups and looked after children.   This vision was further realised and put into place through the National Curriculum for Wales 2008, further emphasising the importance of these key issues that are central to my research.  The document Making the Most of Learning (2008a) clarifies this, suggesting that the†¦ â€Å"†¦development and application of thinking, communication and skills across the curriculum for all learners, schools should choose material that will: provide a meaningful, relevant and motivating curriculum meet the specific needs of learners and further their all-round development. So that the revised national curriculum subject orders and frameworks are truly learner-centred,† (DELLS, 2008a, P4) Legislation promotes the notion that students with SEN should have access to ICT.  ICT is incorporated into the National Curriculum and therefore access should be made to a range of devices to promote inclusion.  Access devices, such as switches, keyboard alternatives, key-guards and joy-sticks can help learners with physical difficulties to use a computer, and enable them to access the same curriculum as their peers.   Pupils, who have literacy difficulties or an impaired visual disability, should also have access to enlarged texts or speech devices and equipment in order that it is possible to hear the words and text in the way that children who do not have SEN, can read without encountering any problems.  For some students technology may be the only way to ensure they can make their thoughts and needs known.  For them, access to appropriate ICT-based solutions possibly provides the only chance of participating in society and realising their full potential. Given the vital role that ICT can play in helping children with special needs to communicate and be involved in learning, it is disappointing that there is relatively little research published in academic journals regarding the use of ICT to support inclusive practice.  Many sources of information include reports from charities and policy organisations with expertise in the area of special needs.  Amongst these groups there are a growing number of small-scale case studies being undertaken (BECTA, BDA), showing the difference that ICT can make to individuals both at school and at home. Many of these case studies are powerful evidence of the potential that technology has in making a profound difference for students.  Such studies may also provide teachers with examples of the use of different types of ICT in varying circumstances, some of which may be applicable to their own students.  Hence even though these case studies may be small-scale, they can be of significant value. The promise that technology brings to education has yet to be truly implemented across all schools successfully which is perplexing due to the strong evidence that permeates throughout educational research and government policy, even though minimal.  There are clearly many obstacles or barriers for schools to progress with the successful application of ICT for supporting their learners, whether this is due to financial support, time, misguidance or even technology overload it is unclear.  Therefore I needed to carry out my own research to investigate the potential of ICT supporting pupils with SEN and share my findings with others to support the development of ICT based pedagogy.   1.2 The Research Organisation and Aims This research will set out to investigate the potential of implementing an ICT intervention strategy to support the learning and development of pupils with special educational needs.  This will be carried out by undertaking an extensive literature review of the current research and recommendations within this field.  This will then be reflected upon, in order to acquire a clear understanding of the possibilities, features and problems related to such an intervention approach.  The information gathered through the literature review will be used to inform a Case Study, focusing on how the implementation of various ICT support techniques could provide an individual pupil, with specific learning needs, improved access to the National Curriculum. In consultation with the school’s SEN team, it was decided that Pupil A would benefit from the intervention strategies, a child with mild/moderate learning difficulties who was receiving one-to-one support 15 hours a week with a Teaching Assistant.  However, shortly after initiating participant training, pupil discussion and implementation of the intervention strategies adopted, an unexpected problem occurred with the whole Case Study.  The parent of Pupil A had been offered a new job which meant that the family had to move out of the area and the school – the research site.  Therefore, the discussion process got underway once more, in the search for a pupil who would benefit from such an intervention process, while being supportive to the research study. I finally decided upon inviting Pupil B to take part in my study, due to the similarities in the difficulties experiencing access to the curriculum as with Pupil A.  Pupil B has been diagnosed with Dyslexia and is currently receiving 15 hours of support per week and is located in the same class as pupil A, therefore the class teacher could still participate. Coupled with this similarity of circumstance for selection, was a point made within Pupil B’s Occupational Therapy Assessment Report (Appendix 10), specifying the recommendation for an ICT intervention strategy in order to support the recording of his thinking and learning. â€Å"As a Year 5 pupil it is important for ****** ‘s long-term recording needs to be developed to permit speed and endurance in order for him to devote his attention to content of work i.e. sentence construction, punctuation, etc.  Development of IT skills and a measured approach to written recording is therefore recommended.† (Appendix 11) This proved to be an ideal solution for the research, though more importantly for the pupil’s needs.  The Pupil Profile section within Chapter 4 highlights the main issues regarding Pupil B’s learning difficulties and the nature of support he requires due to his dyslexia.  Keates (2000) explains that one of the main groups of people with Special Educational Needs who could potentially obtain many benefits from ICT is those with dyslexia. â€Å"Dyslexic pupils face some difficulties in the school including problems in the processing of sound and note-taking. ICT gives access to the curriculum of the subject being taught for dyslexic pupils. Dyslexic pupils often respond positively and quickly to using computer systems, fast realising the support, facilitation and access to a learning environment that ICT affords them.† (Keates, 2000, P4) These are the main reasons for the focus on Dyslexia within this research and the selection of a pupil for the Case Study who possesses this condition. Therefore, coupled with the time frame available and considering the nature of the research site, this selection was deemed the most feasible, in respect to gauging any effect on standards and ability levels through the inclusion of ICT intervention strategies.  In order to measure any improvements a series of pre-test and post-tests will be carried out and comparison made.  Through this approach, an analysis of reading, writing and spelling will be undertaken, which are the main concerns highlighted within his Individual Education Plan and SEN statement. When considering all of these issues two questions were generated in my head which became the Key Research Questions, which act as a guide and focus. Key Question 1: Why adopt ICT in Learning Support for pupils with Special Educational Needs? Key Question 2: How can ICT encourage and facilitate teachers and peers engagement in supportive learning, in a more productive way than might otherwise happen?   These questions are considered throughout the whole research and are reflected on when considering recommendations from literature in the field, examined and discussed within the following Chapter 2.  The research methodologies adopted throughout this inquiry are described in detail in Chapter 3.  While Chapter 4 provides a detailed report of the Case Study carried out with specific reference to the overriding research questions. Finally, Chapter 5 contains a presentation and analysis of the findings exposing the successful outcomes and issues arising from the Case Study.  Conclusions are related and compared with that of claims made by literature within the field in order to justify inferences.  The concluding chapter also offers recommendations for further research and intervention processes for implementing ICT strategies for supporting pupils with SEN. The Potential of ICT Supporting Pupils with Special Educational Needs CHAPTER 2: Literary Review Technology and Pedagogy 2.1 Introduction Although the use of ICT in mainstream education has its origins in the 1970s, it has only been in recent years that the government has identified the importance of and paid special attention to the use of Information and Communications Technology (ICT)  in Special Educational Needs (SEN).  Investment in ICT and the development of policy and practice in meeting SEN requirements have created unprecedented opportunity for the inclusion of all pupils in meaningful learning experiences. This recent and welcomed emphasis on inclusion, coupled with the ever-advancing technologies, have stimulated much interest in using various ICT applications for both individualised learning and for integrating pupils with disabilities into a mainstream school environment.  This chapter provides an overview of some of the issues regarding teaching and learning with technology to support SEN, while exploring the polarized opinions that run through research and literature within this field and the possibilities which these two merging areas within education can provide an individual learner. Davitt (2005), suggest that even though for many decades educationalists and ICT specialists have advocated the potential benefits of using ICT to support and extend learning opportunities, both in mainstream and special education, it is only in recent years that research in this field  is beginning to gain substantial momentum.  Underlying this faith in ICT, whether acknowledged or not, are clear assumptions about the way in which children learn and the attributes of ICT.  The learning theories that are core to most ICT learning to date are considered by Jones and Mercer to, â€Å"†¦embody a strongly individualistic conception of learning which has dominated learning theory and educational practice in this field† (Jones and Mercer, 1993, P19) Many writers have extolled the benefits of using ICT in a learning environment with SEN, suggesting that technology can act as a great equaliser in overcoming or compensating for differences among learners. See, for example, the Code of Practice on Special Educational Needs (DfEE, 1998a), the Green Paper on Special Educational Needs (DfEE, 1997) and the SEN action programme (DfEE, 1998b) which recommends that; â€Å"There will be more effective and widespread use of Information and Communications Technology to support the education of children with special educational needs, both in mainstream and special schools† (DfEE, 1998b, P26) This idea has important implications for learners with disabilities and special educational needs because it suggests that technology can help create the conditions for equal opportunity to learn and equal access to the curriculum for all.  The appeal of technology as an equaliser for learners with special educational needs is borne out in the many materials that have been developed to address special educational needs.  In particular is the formerly National Council for Educational Technology (NCET) now British Educational Communications and Technology Agency (BECTA), who provide a range of information to help identify technologies to aid the learning process of pupils with special needs.  BECTA are the body advising the government on the use of technology in education and published a compendium of research findings entitled ‘IT works!’ (See Appendix: 1) The report made as many as 27 assertions with supportive references from research, however, the assertions made here may need to be seen in the context of a government trying to re-affirm and justify a belief in the educational potential of new technologies.  Nevertheless, they can offer a useful starting point for a discussion of the potential of ICT to enhance pupils’ learning. Professional magazines and trade shows also offer a dazzling array of devices and programmes covering all areas of the curriculum and all types of learning difficulties.  For example, the official magazine of the UK’s National Association for Special Educational Needs, ‘Special’, contains an ICT guide as a regular feature.  This feature explores a range of issues from reviews of programmes to the skills that teaching assistants need to support learners.   It covers all types of learning with technology for all kinds of learners.  Many ICT hardware and software developers such as the Semerc group currently provide training for teachers and support workers to develop their professional practice and provision for pupils with SEN requirements who use their product. 2.2 The Information Supermarket Highway The plethora of available information, software titles and hardware strategies covered under the heading ICT and SEN can be daunting.  In the pressurised world of teaching, there is little opportunity to think critically about what is available or how it should be used and would this best match an individual pupil.  In a review of the instructional effectiveness of technology for pupils with SEN, Woodward et al. (2001) examined the research on software curriculum, specifically designed for pupils with such needs.  They identified a number of design variables thought to affect academic outcomes for pupils with SEN, such as the type of feedback, visual quality, practice, strategy instruction, assessment and motivation. Woodward et al. found that there are no simple answers to the question of effectiveness: â€Å"simply because a program or approach has been validated by research does not necessarily mean it will be used as intended in practice† (Woodward, et al, 2001, P21) The rhetoric accompanying new technological devices in education, and particularly special education, seems to have been very influential, confirming new ways of thinking and talking about teaching and learning.  However, there still prevails a lack of clarity, understanding and application of technology being used to its full potential throughout the education system.   The culmination of grandiose and radical suggestions prominent in commercial slogan and catchy advertisements that are attractive to the educational eye, maybe responsible for our previous lack in informed purchasing, the appropriate matching of resources and effective teaching with the aid of technological resources to promote and maximise the learning of all pupils.   Many government papers are littered with the evidence of mismatched spending and resources for learning, that has resulted in missed opportunities, depleted tax payers finances, and a waste of genuinely keen practitioners time and efforts to provide improved services to their learners and an increased possibility of teachers becoming switched off from the possibilities of ICT enhancing teaching and learning.  The Scottish Government’s paper on Education and Disability (2002) provides a perfect example of this detrimental situation within their plan to improve access to education for pupils with disabilities. â€Å"Through the National Grid for Learning, new computers and networks are being installed in schools across Scotland to allow pupils to benefit from the use of ICT in learning. At the moment, various service providers are being contracted to install the network, but some pupils with disabilities are unable to use these computers for a variety of reasons.  Therefore, as part of their accessibility strategies, responsible bodies should make certain that contracts for any future supply of computers or upgrade of existing stock ensure that the computers (and associated furniture) are accessible or can easily be modified to be accessible to pupils with disabilities. (Scottish Executive, 2002, P 17, 47–48, www 12) What is clear from this financial miscalculation and poor organisation is that the LEA services should be providing schools with the appropriate information for purchasing ICT software and hardware.  Schools should make critical assessments on their ICT requirements in terms of what they want it do, who it is for and what are the expected outcomes from the resource.  Merely placing a PC in a classroom is not going to improve the learning experience for pupils.  Many factors have to be taken into consideration in order for the inclusion of technology to be successfully applied to pedagogy. 2.3 The Technological Pedagogical Debate In early 1998, the Department for Education and Employment (DfEE) responded to the claims made for ICT by publishing a set of criteria to form an integral part of Initial Teacher Training (ITT) courses stating that: â€Å"ICT is more than a teaching tool. Its potential for improving the quality and standards of pupils’ education is significant. Equally, its potential is considerable for supporting teachers, both in their everyday classroom role, for example by reducing the time occupied by the administration associated with it, and in their continuing training and development† (DfEE 1998, P17) This pressure on teachers to assimilate ICT in their work can, therefore, to some extent be seen to be predicated by an acceptance of the claims made in support of the educational potential of ICT.  The potential of ICT to liberate users from routine tasks and empower them, for instance, to focus on the creative and cognitive rather than procedural aspects of writing or to make accessible vast amounts of information is to some extent reflected in the National Curriculum Orders for Information Technology, which emphasise the capabilities of communicating and handling information in various forms. â€Å"Schools should provide opportunities, where appropriate, for learners to develop and apply their ICT skills across the curriculum by finding, developing, creating and presenting information and ideas and by using a wide range of equipment and software.† (DELLS, 2008b, P6) There are clearly strong claims to be made for ICT, but to view ICT as the solution to the educational challenges we face purely by virtue of its sheer existence, is misguided.  The success of ICT use depends on our familiarity with good practice firmly rooted in an understanding of how pupils learn and our reflection on optimal environments of ICT use as bases for pedagogic innovation beyond the assimilation of new technologies into prevailing traditions of classroom practice.  In view of the fundamental changes to our concept of knowledge, the learning process, the role of the teacher and human relations more widely brought about by ICT use, we need to go beyond doing the things we have always done, albeit with the help of new technologies. The core aim of the 1998 DfEE ITT for ICT was†¦ â€Å"†¦to equip every qualified teacher with the knowledge, skills and understanding to make sound decisions about when, when not, and how to use ICT effectively in teaching particular subjects†. (DfEE 1998, p. 17) In my view this aim requires a basic familiarity or relationship with learning theories and the findings from educational psychology as otherwise there is a real danger that the implementation of the computer activity may too easily encourage a distancing of teacher involvement; or as Crook (1994) suggests,   â€Å"†¦a dislocation from the normally rich context of class-based activity and discussion†. (Crook , 1994, P18) Whilst acknowledging the fundamental impact on traditional pedagogical modes, it is important to emphasise how the effectiveness of new technologies in the learning process depends on the ‘centrality’ of the role of the teacher in rendering pupils’ experiences with technology coherent, by embedding them in a context of interpersonal support.  The role of the teacher, therefore, remains pivotal, such as in identifying appropriate learning outcomes, choosing appropriate activities and structuring the learning process.   In their analysis of the contribution new technologies can make to teaching and learning, Gregoire et al. (1996) provided the following with respect to student learning: New technologies can stimulate the development of intellectual skills New technologies can contribute to the ways of learning knowledge, skills and attitudes, although this is dependent on previously acquired knowledge and the type of learning activity New technologies spur spontaneous interest more than traditional approaches Students using new technologies concentrate more than students in traditional settings These positive images are, however, balanced by two further observations of genuine significance: The benefit to students of using new technologies is greatly dependent, at least for the moment, on the technological skill of the teacher and the teacher’s attitude to the presence of the technology in teaching. The skill and this attitude in turn are largely dependent on the training staff have received in this area (Gregoire et al., 1996, P18, www10) Despite the over deterministic inference behind some of the statements, Gregoire et al. (1996) are sounding a warning that technology itself is not a panacea, and that without skilled application by the teacher its benefits may soon recede. The crucial element remains the way in which the technology is incorporated into pedagogical patterns and this is in turn dependent upon the impact it has on the personal theories of the teachers deploying the technology in their classrooms.   2.4 Scaffolding Learning Using ICT Collis et al. (1997) argue that the within a technological approach to pedagogy, the scaffolding role of the teacher is crucial, however the potential of ICT is exploited infrequently due to effective implementation of techniques being heavily reliant on the teacher providing the appropriate support for learning.  Regardless of the suggested gains from any type of technological tool, it is when the teacher supports and guides learning that these benefits are maximised (Waller, 1999).   The computer does not enhance the learning experience unless teachers incorporate ICT very carefully into the curriculum.  The role of the teacher is highly significant in the structure and outcomes of ICT based activities.  The teacher guides and directs the pupils learning through structured planning, organising the activity, interventions during the learning process and the ways pupils apply their ICT skills within various contexts. Mercer and Fisher discuss Bruner’s (1997) idea of ‘scaffolding’, where they suggest teachers need to be reflective and mindful of how they structure learning experience that require the use of technology to support pupil learning. â€Å"If we can describe and evaluate the ways that teachers attempt to scaffold childrens learning with computers then we might be able to help teachers understand and perform their role in supporting childrens computer based activities. â€Å" (Mercer and Fisher, 1997, P210) Bruner (1978) suggests that the Scaffolding process involves the adult guiding and supporting pupil learning by building on previous understanding and abilities.  In assisting the development of pupils, educators require a clear view of learning objectives and understand that their role is to support learners enabling them to develop more independently.  The amount and type of support required will vary depending on the pupil and the nature of the task.  Tharp (1993) put forward a range of strategies that can be adopted to support pupil development through an instructional conversation, described as: Modelling Contingency management Instructing Questioning Cognitive structuring Task Structuring Feedback (Tharp, 1993, P272) According to Tharp, the most productive strategy for support is providing feedback, as this enables pupils to assess their efforts to achieve set objectives, which will be taken into consideration during the planning and participant training phase of this research. Mercer (1993) suggests that the quality of understanding, of which learners obtain through the application of ICT in the classroom, will not be controlled the quality of the technological tool applied; more accurately, it is determined by the approaches utilised to interact between the teacher, pupil and the ‘interface’.  Cook and Finlayson (1999) concur with this idea and describe the application of ICT to support learning as a joint activity, â€Å"†¦the way that learners and the learning support mechanisms of teachers, computer program and fellow group members work together so that the highest possible level of performance becomes achievable.† (Cook and Finlayson, 1999, P100)   In support of this view, Labbo (2000) indicates that relying solely on technology to scaffold learning is not necessarily going to help or maximise the potential of the learner.  Applying a model based exclusively on computer aided instruction is far from ‘authentic learning’; despite the fact that certain educationalists and politicians find this model appealing and the way forward. I believe that before decisions are made to move forward within this field there is a great necessity for further research in order to realise that the combination of technology and how it can support the reciprocal roles of the teacher and child is far more significant than the technology itself.  Arguably, it is this strong pupil-teacher relationship that requires attention and what should be central to the teaching and learning process, even when the technological tool is absent from any learning experience. 2.5 The Potential of ICT Supporting SEN ICT been used to support learners with SEN within mainstream schools for some time, under the terms of assistive or enabling technology, adapting to developments in technology and educational policy changes for learners with different needs.  In Blamires (1999) it is put forward that; â€Å"Ena

Sketchers Advertising :: Business and Management Studies

Sketchers Advertising Advertising is used to inform people about products for sale and to encourage people to buy them. It is a way of telling people about different products that companies are selling. Things are advertised differently depending on whether they are in the newspaper, on television, on the radio or in magazines. The purpose of advertising is to encourage people to buy that particular product rather than another product. There are different forms of advertising. These are: industry and commerce, to sell goods and services and to give information about a certain product. Organizer of event, this is to promote events such as the cinema, theatre, and charity events. Political party, this is to win support on votes, to lobby government. Educational establishments. Which are to advertise colleges and universities. There are also other forms of advertising such as: charity, media, government and private individual. There are also many pros and cons of advertising. The pros are that the advertisements bring the prices down because it encourages competition and increases sales. It gives people information about goods and services. The cons are that it persuades people to spend money on things they don’t really want or need, and they interrupt television programmes, and fill up page after page of magazines. I have looked at the ‘sketchers’ footwear advertisement that I found in a J-17 magazine. The ‘Sketchers’ advertisement is on a plain blue background and takes up a whole double page spread. They have used Britney Spears to advertise the footwear so that people are encouraged to buy them because they think that Britney wears them and they want to be like her. On the first half of the advert there are two pictures of Britney Spears, the ‘Sketchers’ logo, the website address and the words ‘Sketchers USA Footwear’. The website was put on the advert to make people go onto the site and look at more of their shoes. They include these pictures of Britney spears to make the trainers more appealing to the audience to make people try and buy their shoes. On the other half of the advertisement there are two more pictures of Britney Spears. The product is ‘Sketchers’ footwear, the brand name and company is ‘Sketchers’ and there is no slogan. The logo is the letter ‘S’ in a oval shape. The outline of the logo is white, the inside is red and the letter ‘S’ is white. The target audience is teenage girls and women up to the age of about thirty, possibly a bit older. The purpose of this advert is to encourage people to choose their product of

Child Abuse and the Importance of Belonging Discussed in David Pelzers

A Child Called "It" explores the traumatic story of child abuse and how the choices made by one person affects another’s sense of belonging. The story is only from one point of view as it’s a biography, written by the man who experienced trauma at such a tender age. David Pelzer, as a child, dealt with rejection daily and not only from his mother but classmates, teachers and ultimately his biggest enemy, himself. A Child Called "It", as the title denotes depreciation and an impersonal relationship between the audience and the text, begins the biography with a dark and solemn theme as there is no definition for ‘it’ therefore titling a book ‘a child called it’ gives the audience the idea that the child doesn’t exist, or as it tells further in the story, a child who is treated like he doesn’t exist. The author contrasts his mother in different contexts from when his ‘mom’ was a good, caring mum to when his ‘mother’ changed into the abusive stage. Mom and Mother are used throughout the text to show the audience the different emotions he had carried for her. Mom is more personal and informal but suggests a close relationship between the two as it is used in the chapters that he is reminiscing about when his mom was a normal, caring mom. Mother is used in the chapters he was abused in and suggests a more distant relationship due to the formal language structure. When ‘Mom’ was used, the emotions carried out towards her by David Pelzer were none other than belonging to her. When a child is told something so often they start to believe it. In this case, David Pelzer was ordered to say over and over again â€Å"I’m a bad boy, I’m a bad boy...† which psychologically brainwashed him to believe that he was. That every time he was physicall... ...ejected, surrounding him as he says ‘I had readymade excuses mother made for me’. The ending doesn’t end on a ‘happily ever after’ note but rather a dull and gloomy tone as it is set throughout the text. Sarcasm, contrast and similes are the main language features presented in the book to evoke disgust, torment and shock throughout the audience showing them either how she saw the abusive behaviours inflicted upon him or the brainwashed ‘excuses’ he learnt from his mother. Not belonging is the struggle in this text that didn’t get resolved by the ending making it a dark, gloomy tone and the language features used also evokes a dark feeling within the reader. A Child Called "It" truly explores the meaning of struggling with belonging due to other peoples actions rather than their own and provides appropriate use of imagery to emphasise the meaning of the biography.

Process Essay - How to Write a Good Essay :: Process Essays

Process Essay - How to Write a Good Essay I present the following; boiled down into the form of a list it may be easily understood by all teachers of writing, even those whose first name is for all intents and purposes "Coach." 1. Get a sheet of paper. This law is thought by some to have been superceded by the advent of computer technology. However, it is still an important first step in that it leads to the following: 2. Sit at a desk with the paper on it, scribbling senseless doodles, silly faces, and, if you are so inclined, the name of an object of affection surrounded by hearts, flowers, and, again if you are so inclined, barbed wire. 3. Start feeling guilty for wasting so much time and begin staring off into space waiting for the muse to start blowing you kisses or for inspiration to shoot a lightning bolt through the top of your head. 4. Hum. 5. Begin tearing pieces of the paper off and put them in your mouth. Chew. Swallow if necessary. 6. Now that you are ready to write your first draft, turn on your computer and/or word processor and begin cruising the Internet or wiping dust off of your screen with the aforementioned paper or your thumb, whichever is more convenient. 7. Remember that you've done no research. Go to the shelf and pick up a volume of the World Book Encyclopedia, preferably "P-R." "Q" always has some interesting topics in it. 8. Finally settle--after combing through a few more volumes--on something to do with Wombats. 9. Remember that the instructor actually gave you a list of possible topics from which to choose. 10. Search in vain for the list of possible paper topics your instructor gave you from which to choose. 11. Call up your friend who is also taking the class and beg her for the list. 12. Meet for coffee. Forget about the list. 13. Call her the next day and ask her to just read a few of the topics over the phone to you. 14. Listen in horror as she says: "You did know this paper is due tomorrow, didn't you?" 15. Go to the library. 16. Research by checking out randomly selected books on vaguely related topics. 17. Highlight like mad. 18. Look at the stack of books for a good long while.

Conducting a Strategic Management Project Essay

Introduction Shell is one of the world’s leading energy group and petrochemicals companies. It has around102000 employees in more than 100 countries. The objectives of the Shell Group are to engage efficiently, responsibly and profitably in oil, oil products, gas, chemicals and other selected businesses and to participate in the search for and development of other sources of energy to meet evolving customer needs and the world’s growing demand for energy. Mission Statement Shell’s innovative approach ensures that they are ready to tackle the challenges of new energy futures. 1.1 Topic of investigation having a strategic implication. This report explains the team buildings and teams work in Shell and development of its research work progress within the organization. It also presents suitable analysis of the project management and planning, advisory and project implementation of work. In this report, positive results of theories and its test showed that effective teamwork can lead to a success and achieving critical goals of Shell. It may also be able to gain the sustainability of competitiveness. Moreover, study explains that how team performance can be improved through leadership with project planning to face the upcoming challenges in modern era of business environment Shell operates in. 1.2 The aim, scope and objectives of the project. †¢ How can we improve Shell’s research study about lubes oil? †¢ What strategy we should adapt for improving Shell’s research? †¢ How and effective team force can be made for this purpose? The aim of the project is to achieve such team management, technology and resources that meet the new emerging energy challenges. Shell need to adapt such management strategies which will make a strong workforce with experienced and skilful team members. Shell wants to be recognised as a great company-competitive successfully and a force for progress. Shell has a fundamental belief that they can make a difference in the world because of it’s worth in the global market. A strong consumer proposition has been developed for the brand and embodied in the line â€Å"World Quality† which communicates Shell value-based offering in a precise manner, to its core target audience. The purpose of the advertising in the first instance is to support this strong value pr oposition and secondly to tailor the media solution to the store. 1.3 Justifying the scope, aim and objective. In meeting the energy challenges, the world faces three hard truths. The first is that there has been a step-change in global energy demand, with rapidly developing countries like China and India entering the energy-intensive phase of growth. Even with huge improvements in energy efficiency and substantial growth in renewables, fossil fuels will still be the main element of the energy mix by mid-century. The second hard truth is that easy-to-access oil and gas is in decline. As a result, energy will come increasingly from unconventional sources, such as oil sands. The third hard truth is that the increased burning of fossil fuels especially coal for power generation could mean unacceptably high emissions of carbon dioxide (CO2) and other greenhouse gases that contribute to global warming and climate change. Meeting the energy challenge Shell is helping to meet the energy challenge with a broad range of approaches. It uses advanced technologies that can unlock oil and gas in more remote or hostile environments, and new techniques to extend the lives of existing fields. We are increasing production from unconventional sources, including oil sands. Shell helps make the most of cleaner-burning natural gas through our output of liquefied natural gas (LNG) and gas to liquids (GTL) products. We are helping to develop second-generation bio fuels that do not compete with food crops; and we are developers of wind and solar power. 1.4 Evaluation of project research methodology. Methodolgy Research Study should be verified by seniors because all research is based on practical data and that data may vary by location of the research and by person. Need to Understand the Hypothesis before research and development. Which department are more feasible and which person is more interested and suitable for that research. Research should be complete on time. Research conduct at same time on different locations 2.1 Identify sources of data and information that will support the aim of the project. For a company like Shell having the right information about the environment, customs, laws and customer needs is of key importance. To gather this information the company will firstly see the law of the country in which it is going to start business, as we are talking about their business in UK the law allows this business. Here in UK there is a high demand it is a rich environment for the company to run their business. Data about this can be obtained by doing surveys and examining the current market i.e. by observing the similar businesses running in the country. Scanning the Environment In this research study, an appropriate approach of environmental scanning is considered in the industrial organisations that are operating in the chemical industry. Different types of information were used in this study. It included general information about the industry and specific about the participating companies. The type of data that was included was; †¢ Formal data that concerns the company’s character. †¢ Data that is publicly available, like in annual reports. †¢ Interviews of managers that provided the history and culture of the companies. This information was very important to analyse and research the topic and to see how other companies are operating in the industry. Strauss (1987) emphasizes the usefulness of the case study approach when used with grounded theory. Grounded theory seeks to generate theoretical statements and, ultimately, complex theories based on empirical evidence, although it can be used in different ways and reach various degrees of complexity. The research design framework adopted in this study can be described as a multiple case study, composed according to the theory building structure, as described by Yin (1989), i.e., where the sequence of chapters follow a theory-building logic, and using the grounded theory method of qualitative data analysis. 2.2 Synthesise the data and information for options or alternatives that support the project aims. An effective information infrastructure was implemented to assess the information required in this research, which included technological information, processes and information acquired by people. A rich collection of scientific and technical information was gathered, which was managed by professionals having different backgrounds. However, some companies just provided a consistent picture which was a small collection made up of journals, literature and reports mainly. The information being pervasive is one of the main reasons in having difficulty in accounting for the costs involved in the research as it depends on specific roles and their performance. The data that was collected showed that in medium or large chemical companies data and information was being handled by 50% staff while in smaller companies about 9% staff was involved. The scope of the companies was assessed by their li nks with Research and Development organisations and hoe they collaborate with the regulatory agencies. Strategic change Larger companies like Shell influence the planning adoption but some other factors also interfere with the tendency, for example in what form the organisation is and what is the management style of the organisation. No evidence was found that suggests that the subsector companies should adopt the planning technique as a planning tool. While, the planning offices are also rare and their main duty is to collect the difficult data which is needed by the top management for decision making. The strategic change analysed mainly talked about increasing the quality of the product, which includes mainly improving the conditions of production. Environmental protection was also an issue in some cases there were highly pollutant industries. Globalisation and diversification were also some important strategic changes. Companies that targeted globalization pursued growth in the industry but some companies just secured their positions in the internal market due to the threat of major competitors operating in the main market. Companies which had a little scope of growth adopted specialization. The main target was to improve the product quality. Some companies claimed that they always pursue improving the production quality while other companies admitted that they have to improve the quality of the product to satisfy the EC regulations. 2.3 Determine an option or alternative that supports the project aims. Other way of supporting the project aim is to use theoretical information and implement it directly on the company. Information is like team management theories. Inductive and deductive research can be used to gather information and to move towards the aim. Deductive research Deductive reasoning works from the more general to the more specific. Sometimes this is informally called a â€Å"top down† approach. Conclusion follows logically from premises Inductive research Inductive reasoning works the other way moving from specific observations to broader generalizations and theories. Informally, we sometimes call this a â€Å"bottom up† approach. Conclusion is likely based on premises..It involves a degree of uncertainty. Inductive type based on induction. Data is collected on a subject and researcher tries to develop theory from this data. 3.1 Evaluate the research analysis to enable conclusions to be made. Team Management It is difficult to build a team and maintain good working relationships. However, team members go through a number of development stages to work together effectively in team performance management. Individuals performing well are rewarded while those who are not performing good are motivated. The functions and roles of its members should be recognised by its team. The leader should establish continuous communication lines and set clear goals that are understood. A team that performs well accomplishes projects quickly and with less difficulty, misunderstanding, and divergence. Strategic Objectives Strategic goals or objectives are broad, high level aims, derived from organisation’s mission statement and purpose, affecting the whole organization (sometimes referred to as corporate aims). These will be: †¢ Long term (time horizon of several years), although they should be monitored regularly (typically annually) and may be revised periodically. †¢ Responsive to external factors such as new competition, changes in global economic conditions, or customer demands. The term ‘strategic’ denotes a particular focus of the managers’ planning and decision making responsibilities. Senior mangers focus mostly on ‘why’ questions, concentrating on the ‘big picture’ and providing vision and leadership for employees across the whole organisation. Alignment of team performance and corporate goals To achieve the corporate aims it is necessary that the direction of the teams is towards the long term strategic goal. Managers and team leaders have to keep in mind what they need to achieve overall. And hence, to control the teams in such a way that either they are working on long projects or short it’s all adding up to the final goal. In the case of Shell the main objective of the company is to provide good quality chemicals. To achieve this there has to be a proper direct and control system of the staff working at the stores from the top management to the staff working on the floor. A good communication and understanding will always lead to easy achievement of goals, satisfying the customer at the end. Mangers are supposed to take into account the demand and supply of the products to make sure every product is available on time and then work should be allocated to the subordinates in a proper system to get all the work done systematically and easily. Proper team management should be done by the supervisors and the team leaders. All the staff should be divided into small teams which have to work in different sections. Proper training should be given to all the team members and a standard way of achieving the goal. This performance target should be given to all the team members and should be monitored and reviewed constantly. This standard should be according to the company policy and should be according to health and safety and customer satisfaction oriented. Providing good work at the end of every shift, which is the availability of all the products on time with good customer service. 3.2 Recommend a course of action that achieves the project aim. Skills that need some development †¢ Communicating †¢ Decision-making †¢ Leadership †¢ Prioritizing †¢ Valuing and †¢ Skills need to be achieved. †¢ To respond problems, experiences and opportunities from which to learn. †¢ To generate ideas without constraints of policy or structure or feasibility. †¢ Involving with other people i.e ideas bouncing off them, solving problems as part of a team. †¢ Need to have a limelight/high visibility i.e can chair meetings, lead discussion, and give presentations. †¢ To be engaging myself in short, activities such as business games, competitive teamwork tasks, role playing exercises. Supporting Others †¢ Motivating †¢ Analysing †¢ Delegating †¢ Reporting 3.3 Analyse the impact of the recommendations. Training courses at Shell for career progression. Management Development Programme Includes: †¢ Consumer Legislation †¢ Customer Service †¢ Employment Law †¢ Health and Safety †¢ Human Resource Management †¢ Policies & Procedures Opportunity development builds competitive positions by identifying and utilizing opportunities in the environment. Opportunity development is necessary because your existing positions are constantly being degraded by change. Opportunities exist as â€Å"openings† in the environment that allow you to advance your position in the direction of your mission. These openings are usually small, but by taking advantage of the small openings, you eventually position yourself for the big advances you want. Training After the initial audit our ethical trade specialists work with the suppliers to support them through remediation of any issues that are found. Training is offered to suppliers on particular issues and best practice, at regular intervals. Shell is in the process of more than tripling the size of its own ethical trade team that focuses on training and intensive problem solving with its suppliers. These individuals are trained to the highest levels and are equipped to address endemic problems in the supply chain and come up with viable solutions for the suppliers. 4.1 Produce the results of the investigative project. The purpose of this study was to explain the team buildings and teams work in Shell and development of its research work progress within the organization. It also presents suitable analysis of the project management and planning, advisory and project implementation of work. In this report, positive results of theories and its test showed that effective teamwork can lead to a success and achieving critical goals of Shell. It may also be able to gain the sustainability of competitiveness. Moreover, study explains that how team performance can be improved through leadership with project planning to face the upcoming challenges in modern era of business environment Shell operates in. The main result was in the adoption of the learning and training courses within the company in order to prosper and enhance in the future. Shell is one of the leading companies in the world in the chemical an oil sector and it felt pride in taking these training and development steps in order to move forward and to achieve its project aim as a result of the research program. 4.2 Evaluate the impact of the investigative project The future sustainability of any organization heavily depends on the quality of project management to be able to cope with today’s dynamic business environment. There is direct co relation between stake holder business project and the people within the firm. Shell is one of the four largest companies in the UK and has been ranked number one of the top 100 graduate employers in the UK and all over the world. The sustainability of Shell competitive advantage of its brightest people is depending on the successful team work and leadership. The best leaders know how to get others to follow and the best team members know how to follow their leaders. REFRENCES BOOKS: Aguilar, F.J. (1967) Scanning the Business Environment. New York: McMillan. Robert , buttrick ,(2005).The project workout: pearso education limited Edinburgh gate Education 3rd (1) pp, 305-350 Strauss, A. & Corbin, J, (1990). Basics of Qualitative Research: Grounded Theory Procedures and Techniques. London: Sage. Strauss, A. (1987) Qualitative Analysis For Social Scientists. Cambridge: Cambridge University Press. Total Quality Management: The route to improving performance by John S. Oakland (Hardcover – 15 Mar 1993) Shell employee control hand book 07/08 Personal development as a strategic manager unit 7001: level 7 strategic management and leadership CMI Checklist: †¢ Personal development planning †¢ Developing strategy for world class business E-learning †¢ Your role in improving personal effectiveness †¢ Development needs and planning development Web links: †¢ www.milum.net 13/12/2009 †¢ www.enotes.com 13/12/2009 †¢ www.quickmba.com 13/12/2009 †¢ www.managers.org.uk 10/12/2009 †¢ www.shell.co.uk 20/12/2009